negative staining


negative staining
Microscopic technique in which the object stands out against a dark background of stain. For electron microscopy the sample is suspended in a solution of an electron-dense stain such as sodium phosphotungstate and then sprayed onto a support grid. The stain dries as structureless solid and fills all crevices in the sample. When examined in the electron microscope the sample appears as a light object against a dark background. Quite fine structural detail can be observed using negative staining and it has been used extensively to study the structure of viruses and other particulate samples.

Dictionary of molecular biology. 2004.

Look at other dictionaries:

  • negative staining — n a method of demonstrating the form of small objects (as bacteria) by surrounding them with a stain that they do not take up so that they appear as sharply outlined unstained bright bodies on a colored ground * * * staining of the background and …   Medical dictionary

  • negative staining — negative staining. См. негативное окрашивание. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • negative staining — noun : a method of demonstrating the form of small objects (as bacteria) by surrounding them with a stain that they do not take up so that they appear as sharply outlined unstained bright bodies on a colored ground * * * negative staining, the… …   Useful english dictionary

  • negative staining — A staining procedure in which a dye is used to make the background dark while the specimen is unstained …   Dictionary of microbiology

  • Staining — is an auxiliary technique used in microscopy to enhance contrast in the microscopic image.In biochemistry it involves adding a class specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a… …   Wikipedia

  • Negative stain — Electron micrograph of (organic) plant tissue without (top) and with OsO4 staining Negative staining is an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid. For bright field… …   Wikipedia

  • Gram staining — (or Gram s method) is an empirical method of differentiating bacterial species into two large groups (Gram positive and Gram negative) based on the chemical and physical properties of their cell walls.cite book | last = Bergey | first = David H.… …   Wikipedia

  • Gram-negative bacteria — Microscopic image of Gram negative Pseudomonas aeruginosa bacteria (pink red rods). Gram negative bacteria are bacteria that do not retain crystal violet dye in the Gram staining protocol.[1] In a Gram …   Wikipedia

  • Gram-negative — bacteria lose the crystal violet stain (and take the color of the red counterstain) in Gram s method of staining. This is characteristic of bacteria that have a cell wall composed of a thin layer of a particular substance (called peptidoglycan).… …   Medical dictionary

  • Differential staining — is a general term that can refer to a number of specific processes. Generally, it is used to describe staining processes which use more than one chemical stain. Using multiple stains can better differentiate between different microorganisms or… …   Wikipedia


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